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While the carrier for the combination of benfotiamine and pyridoxamine can consist of a relatively simple solvent or dispersant such as an oil, it is generally preferred that the carrier comprise a composition more conducive to topical application, and particularly one which will form a film or layer on the skin to which it is applied so as to localize the application and provide some resistance to perspiration, and/or one which aids in percutaneous delivery and penetration of the active ingredients into lipid layers

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Glutathione S-transferases (GST) conjugate GSH to drugs and drug metabolites, thus facilitating drug-conjugate elimination and efflux

Chemical standards were prepared in ultrapure water and were analysed under identical conditions as samples to validate metabolite identities by chromatographic retention time and MS/MS fragmentation pattern matching