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The main reaction steps for the preparation of silver nanoparticles using ethylene glycol reduction of silver nitrate are as follows: Figure 2 As shown in Figures 2C,D for the high-resolution TEM images of AgNPs, it is calculated that the crystalline plane spacing of the adjacent lattice is 0.2375 nm, which is close to the lattice constant (0.2358 nm) corresponding to the Ag (111) crystalline plane in the PDF card, so the AgNPs grow mainly along the (111) direction

The following day, cells were washed three times with DPBS and incubated with the corresponding secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 555 fluorophores (Thermo Fisher Scientific, Inc.) for 2 h at room temperature

It's even more critical if you're pregnant or planning to become pregnant

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