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To highlight cell thrombin generation dependence on TF, the CAT assay was performed after pre-incubation of cell samples with an inhibitory anti-TF antibody (AD #4502, polyclonal rabbit anti-human TF, American Diagnostica Inc., Stamford, CT, USA).16 A non-specific antibody (Rabbit IgG fraction, Dako, Denmark) was used as the control in this assay

Visual inspection catches gross contamination cloudiness, particulates large enough to see, discoloration, seal integrity failure

Therefore, we consider that the regulation of intracellular iron ion content by radiation to cause iron overload to enhance ferroptosis sensitivity in tumor cells is expected to be an effective way to enhance radiosensitivity

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