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5 A recent cohort study of NAD+ and a proprietary combination of B vitamins, amino acids, and DL-phenylalanine (an enkephalinase inhibitor) IV for substance abuse disorder found statistically significant improvement in cravings, anxiety, and depression scores for patients on IV NAD+.5 Another study on patients with alcohol or opiate addiction found doses of 500-1500mg per day (in combination with some vitamins, amino acids, and n-acetylcysteine) for 10 days to reduce cravings and number of relapses when patients were surveyed 12-20 months post treatment

Mechanistic Studies Subsequent research has explored the mechanisms by which NNMT regulates metabolism, with studies demonstrating that NNMT activity reduces cellular NAD+ availability, affects SAM-dependent methylation reactions, modulates sirtuin activity, and influences adipocyte differentiation and function