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We refer readers to the FY 2013 IPPS/LTCH PPS proposed and final rules for additional background and a detailed description of this methodology (77 FR 28145 through 28146 and 77 FR 53700 through 53701, respectively)

MiR05 medium (0.5 mM EGTA, 3 mM MgCl 2 .6H 2 O, 60 mM K-lactobionate, 20 mM taurine, 10 mM KH 2 P0 4 , 20 mM HEPES, 110 mM sucrose, and 1 gl 1 BSA) was used to assess respiration