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In conclusion, although there is substantial evidence in animals that numerous selenoproteins (e.g., mGPX4, nGPX4, GPX5, TXNRD3, selenoprotein P) are crucial for normal male reproductive function, and that their deficit (genetic knockdown) leads to reduced or total infertility, the studies in humans are still scarce

Bulletin of Experimental Biology and Medicine , 164 (1), 4143

Sperm suspensions were then homogenised by careful flicking of the Eppendorf tube, and a sub-sample was diluted 1:4 in milli-Q water (10L of thawed treatment sample in 40L of milli-Q water) to activate sperm by lowering osmolality

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