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It should be noted, however, that there are other molecules and pathways that are relevant to SG fibrosis, although they have been previously only briefly mentioned in the immune cell section

An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)