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12 Briefly, after genomic DNA extraction and quantification by PicoGreen fluorescence (Invitrogen, Carlsbad, CA, USA), the DNA was used as template for the PCR reaction

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[254] There is growing appreciation of the importance of the pleiotropic hormone vitamin D in the development of tolerance, immune system defenses, and epithelial barrier integrity [255] 1,25(OH)2D3 (vitamin D) may play a protective role in mucosal barrier homeostasis by maintaining the integrity of junction complexes and in healing capacity of the colon epithelium