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In general, the root architecture is regulated by a various of signaling molecules, such as Ca 2+ , ROS, NO, H 2 S, Glu, and plant hormones (ABA, auxin, and cytokinin), as well as their crosstalk ( Arabidopsis , iGluR competitive antagonists [6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 6,7-dinitroquinoxali-ne-2,3-dione acted (DNQX)] dramatically inhibited the growth of primary root and the density of lateral root by impairing root meristem size

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These findings underscore the potential of melatonin as a valuable supplement in IVM and in vitro growth (IVG) protocols, thereby improving reproductive outcomes