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Metabolomics studies have further demonstrated that EGCG exerts anti-lung cancer benefits by regulating 33 differential metabolites in A549 cells, which are involved in various metabolic processes including glucometabolic, amino acid metabolism, nucleotide metabolism, and vitamin metabolism [596, 597]

Real timePCR Total RNA of the cells was extracted using TRIzol reagent (Invitrogen), the RNA was converted to cDNA and the RTPCR was performed using NGF gene-specific primers (Supplementary Table 2) and the 7900 Fast RTPCR system (Applied Biosystems) to determine NGF mRNA expression