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A single-cell RNA-Seq (scRNA-Seq) study analyzed primary NP and AF cells isolated from healthy adult discs and observed that receptor signaling pathway genes were highly expressed in the AF, whereas protein synthesis and ECM genes were highly expressed in the NP (Fernandes et al., 2020)

Purified IgG variants were concentrated using Amicon Ultra 50 K spin columns (Merck) and stored in either 1x phosphate buffered saline (PBS) (NIP, anti-CD20, anti- S

In untreated women, a further decrease in ferritin level and increase in anaemia occurred ( Reference Nekrasova, Strongin and Ledentsova 49 )