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These ELISA positive clones may also be further characterized by techniques known in the art, such as, for example, epitope mapping, binding affinity, receptor signal transduction, ability to block or competitively inhibit antibody/antigen binding, and competitive agonistic or antagonistic activity

5.4 Gut microbiota metabolites and lung immunity In addition to SCFAs, bile acids (BAs), tryptophan metabolites, and others, as key molecules of gut microbial metabolism, are involved in the regulation of immune modulation and organ damage through multiple pathways in sepsis