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Conclusion Nickel-NTA affinity chromatography is an effective and widely used technique for purifying recombinant proteins, and the His6 tag is the most common tag used for this purpose

It is also crucial to support low-income countries

However, it remains unclear, what precisely affected the development of the oxidative stress-induced apoptosis while changing the level of Grx5 (overexpression or knockout of the gene): alterations of the thioldisulfide homeostasis or homeostasis of the ironsulfur clusters

Gene delivery In vitro and in vivo studies have proven the efficacy of IGF-1 gene therapy in OA treatment [16, 60, 61]