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BPC-157 has been studied extensively in preclinical models and has demonstrated meaningful effects on tissue repair, tendon and ligament healing, muscle recovery, wound healing, angiogenesis, nitric oxide signaling, inflammation modulation, and gastrointestinal mucosal protection

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Following cell attachment, the medium was replaced by 0.5 ml of serum-free cell incubation medium (HI medium, BioIVT) per well and pre-cultured overnight at 37 C in an incubator