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Moreover, chondrocyte apoptosis, triggered by ER stress, nitric oxide, and oxidative insults, plays a critical role in cartilage degradation

Am J Physiol Gastrointest Liver Physiol 2003;284G385-G391.Article 157

the siRNA compound is sufficiently large that it can be cleaved by an endogenous molecule, e.g., by Dicer, to produce smaller siRNA compounds, e.g., siRNAs agents the sense and antisense strands may be chosen such that the double-stranded siRNA compound includes a single strand or unpaired region at one or both ends of the molecule

Cells were homogenized with 50 strokes using a Dounce homogenizer on ice and then centrifuged at 600 g for 10 min at 4 C