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Description

Bibliometric analysis of global research trends on ultrasound microbubble: A quickly developing field

Cells were lysed in modified RIPA buffer (50 mM TrisHCl, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1 mM -mercaptoethanol, 1% Igepal, 0.5 % sodium deoxycholate, 0.1% SDS, 1 mM phenylmethylsulfonylfluoride (PMSF), 1 mM Na 3 VO 4 , 1 mM NaF, and fresh protease inhibitor cocktail)

139 ShyrS

The resulting mixed disulfide between Trx and the substrate is reduced by the more C-terminal active site cysteine, thus completing the exchange reaction