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The presence of excess methionine results in global gene expression changes, with a unique anabolic transformation signature (Walvekar et al., 2018)

Following this, the coverslips were blocked with 5% BSA in PBS for 1 h at room temperature

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High intensity blue light can cause irreversible inhibition of cytochrome oxidase, damage mitochondrial function and trigger cell apoptosis, while reducing sodium potassium ATPase activity, causing ion imbalance and increased osmotic pressure inside and outside the cell, leading to cell edema and organic damage, ultimately causing photoreceptor degeneration (Qin et al., 2019)