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Furthermore, mutations in mitochondrial and nuclear genes encoding proteins that are required for proper assembly and stability of the mitochondrial respiratory complex also lead to complex I deficiency

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During ischemia, oxygen deprivation suppresses critical metabolic pathways such as those involving fatty acids, ketone bodies, branched-chain amino acids (BCAAs), and glucose oxidation, drastically reducing ATP production

Reads were trimmed from the 3 end where the Phred quality score fell below 20, and sequences shorter than 20 nucleotides after trimming were discarded