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When p62 is knocked down via RNA interference, the expression of NQO1, HO-1 and ferritin heavy chain is decreased, exacerbating erastin-induced ferroptosis (45,46)

10.1016/j.ajme.2017.09.001 241

Real-time PCR was performed using 2x Universal SYBR Green Fast qPCR Mix (Abclonal RK21203) on a CFX Duet Real-Time PCR System (Biorad)

Lipid peroxidation, ferrous iron and GSH measurement For lipid peroxidation analysis, the cells were seeded in six-well plates one day before treatment