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Total RNA was isolated using Qiagen RNeasy mini kit (Cat # 74106), RNA sequencing libraries were prepared with Illumina-compatible NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) were carried out by Genotypic Pvt.Ltd

They: Produce collagen Organize extracellular matrix Restore tensile structure In experimental tendon transection models, BPC-157 has been associated with: Increased fibroblast density Enhanced migration into injury site Accelerated granulation tissue formation Proposed signalling involvement includes: FAK (Focal Adhesion Kinase) activation ERK1/2 signalling Src pathway modulation FAK signalling is particularly important because it regulates: Cell adhesion Cytoskeletal reorganization Migration along collagen scaffolds Tendon strength depends not just on collagen quantity but alignment

Proceedings of the New Zealand Society of Animal Production, 62, 307-310