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Membranes were then incubated overnight at 4C with the following primary antibodies: NINJ1 (1:1000, Invitrogen, PA5-95755), GAPDH (1:1000, Cell Signaling, #97166), PANK1 (1:1000, Cell Signaling, #23887), xCT (1:1000, Cell Signaling, #12691), V5 tag (1:1000, Cell Signaling, #13202), and -tubulin (1:1000, Cell Signaling, #2128)

The duration of storage may also affect the physical and chemical properties of fermented mushroom such as color change, decreased pH value, and reduced anthocyanin content (Nurerk et al., 2022)