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Tumours and tissues were snap-frozen on dry ice and stored at 80 C or placed in 10% neutral buffered formalin (Fisher Scientific, 22-110-761) for 24 h and then stored in 100% methanol (Fisher Scientific, A412P-4)

Nature, 491 , 364373

The first category included adenovirus overexpression plasmids for wild-type and mutant human TXNDC5 and USP2 genes, with restriction enzyme sites AsisI/MluI, a 3Flag tag fused at the C-terminus of the vector, and an Amp resistance gene (resistance concentration: 100 g/ml)

[34],[35] After milk consumption for 1 month, children have shown to have a higher mean plasma level of IGF-1, higher IGF-1 / IGFBP-3, and GH levels