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10.1186/s12967-015-0536-7 J Transl Med

The treatment can be helpful in reducing symptoms of reflux

Analysis of dissociation curves was used to confirm the identity and specificity of PCR amplicon product without primer-dimer using its expected melting temperature (Tm), where after the end of the PCR experiment, the temperature was gradually raised from 65 C to 95 C to conduct the dissociation melting curve study