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The freeze-drying process already removed the water, so ice crystals cannot form

The resulting clean reads were assembled into unigenes using Trinity software (v2.13.2) after Q20, Q30, GC-content and sequence duplication level of the clean reads were calculated

The test is based on the idea that AST, an enzyme that needs pyridoxal phosphate (PLP), moves an amino group from aspartate to -ketoglutarate, which makes oxaloacetate and glutamate

In terms of neurotransmitter regulation, probiotics modulate microbial metabolism in the gut, influence tryptophan metabolic pathways, increase serotonin synthesis, and ameliorate neurodevelopmental disorder symptoms (Yano et al., 2015)