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In addition, the patients in this study with more severe disease were most likely simultaneously receiving multiple therapeutic interventions, which may have confounded some of the findings in this group

In our study, MTX was shown to significantly elevate MDA levels while reducing GSH levels in liver tissues, contributing to oxidative stress and the formation of lipid peroxides as observed in other studies

Recognising these interconnected symptoms enables timely intervention before deficiency leads to permanent damage

Another limitation is that the peptide half-life chart lists plasma half-life, which is the pharmacokinetic measure most commonly reported in the literature