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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

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Chemical shifts were referenced to the internal 3-(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) signal for 1H 1D spectra

More studies are needed to better understand the role of FOXO in the pathogenesis of neurodegenerative diseases and its potential as a therapeutic target for PD treatment