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GAPDH (glyceraldehyde 3-phosphate dehydrogenase) served as a loading control for total protein, while Histone-H3 was used for nuclear extracts

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Confocal microscopy analyses showed that nucleolar NPM remains phosphorylated specially in p-NPM(Y29), p-NPM(Y67), and p-NPM(Y271) in basal conditions and during its translocation to nucleoplasm, which occurs after cell irradiation with UV (Figure 3BD and Supplementary Figure 3BD)