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Annu Rev Physiol (2012) 74:6986

Full-length human GSK3 cDNA was amplified by PCR from a previously described plasmid encoding HA-GSK3 49 and then subcloned into the Eco RI and Sal I sites of the pCS3+MTBX vector or the Eco RI and Nco I sites of the pCS5-Flag vector to yield 6xMyc-GSK3 or Flag-GSK3