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Maccarrone M, De Petrocellis L, Bari M et al (2001) Lipopolysaccharide downregulates fatty acid amide hydrolase expression and increases anandamide levels in human peripheral lymphocytes

After 30 minutes of incubation at 37 C in the dark, the percentage of PI positive cells was determined by using a flow cytometer (BD FACSCanto II, BD Biosciences), with 488 nm excitation laser and 585/42 nm band pass filter