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Description

Following incubation, the beads were washed with lysis buffer, and the proteins were eluted with GST elution buffer (75 mmol/L Tris-HCl pH 8.0, 20 mmol/L-Glutathione) and His elution buffer (50 mmol/L NaH 2 PO 4 , 300 mmol/L NaCl, 250 mmol/L imidazole, adjust pH to 8.0), respectively