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Subject terms: Biochemistry, Cell biology, Metabolic disorders Introduction Nicotinamide adenine dinucleotide (NAD + ) is an essential redox coenzyme that exists between the oxidized form NAD + and its reduced form (NADH) 1

differentiated 3T3-L1 adipocytes could be utilized as a cell-based system for mechanism-of-action and phenotypic characterization of NNMT inhibitors NNMT protein expression was found to be 37-fold higher in the adipocytes (day 9) vs pre-adipocyte (P 0.0001)

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A clinician then removes the needle, applies light pressure and disposes of the sharp safely in a sharps bin