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Furthermore, initiation of treatment at a later time would mean that the untreated (control) mice would have too big a tumour burden to survive the duration of the experiment (a situation unacceptable to our Animal Ethics Committee)

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Medications for obesity: a review

The most frequently used tracers are lucifer yellow (LY: 444 Da), fluorescein sodium (NaF: 376 Da) or sucrose (342 Da), and fluorescent dextran (70 Kda) for larger molecules