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Why Accurate Peptide Calculation Matters for Research Manual dosage math introduces variables that compromise research reproducibility

SDS-PAGE and Western blotting To compare AlphaLISA with western blotting detection, the bulk transfected cells were seeded into either 384-well (for AlphaLISA analysis) or 24-well plates (for western blotting analysis) at 25% confluency in parallel and treated with or without PIs as indicated