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[DOI] [PMC free article] [PubMed] [Google Scholar] 48.Du D, et al

In Vitro S-Adenosyl-L-methionine (300 M, 24 or 48 h) chloride dihydrochloride induces cell apoptosis, and promotes the cell cycle arrest in Cal-33 and JHU-SCC-011 cells [4]

Cells were fixed using 10% buffered formalin for 10 min, then permeabilized in blocking buffer (10% serum, 1% Triton X-100, in 1X PBS) for 1 h, then primary antibody diluted in blocking buffer was applied overnight (See Table S4 for antibody concentrations)