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Enzyme activity determination Candida albicans cells were grown in SD liquid media until the early (12 h) and late (24 h) exponential phases, harvested by centrifugation at 6,000 g for 15 min, and homogenized using a Mini Bead-Beater (Biospec Products) for 90 sec con- taining 1 mM phenylmethanesulfonyl fluoride (PMSF)

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Bacteriostatic water is commonly used as a sterile diluent in controlled research laboratory settings

Recently, these long rod-like proteins, including ATG2 and VPS13, comprised of predicted anti-parallel -strands-containing repeated modules, have been referred to as RBG proteins (Neuman et al, 2022)