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For experiments in 6-well plates, 50,000 cells were seeded, and after 24 hours, the medium was aspirated and washed once with PBS, and the indicated medium was added

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But its clinical efficacy is not ideal and glioma resistance to TMZ is the most important reason for chemotherapy failure

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These results complement earlier studies showing anti-oxidant effect of MSM and suggest that single dose oral supplementation with MSM lowers exercise induced oxidative stress in healthy untrained young men, but is not adequate to significantly affect plasma GSH level