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13 C 6 -Glucose tracing metabolomics Cells were cultured in glucose-free RPMI-1640 (Teknova) media supplemented with 2 g/L 13 C 6 -glucose (Sigma) and 10% dialyzed serum (Life Technologies, A3382001)

Cells were selected with 2 g/mL puromycin 24 h post-infection and selected for 72 h

Additionally, copper is required for the absorption and utilization of iron, another nutrient found in beef liver that is important for overall gut health