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The active RAGA GTP RAGC GDP Ragulator complex was recruited to LUVs containing the lipid DGS-NTA(Ni) by means of a 6His tag fused to the N terminus of the LAMTOR1 subunit of Ragulator in place of the physiological myristoyl and palmitoyl modifications (Fig

Editor information Editors and Affiliations Rights and permissions Copyright information 2021 The Author(s), under exclusive license to Springer Nature Switzerland AG About this chapter Cite this chapter Arif, Y., Singh, P., Siddiqui, H., Hayat, S