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So there could be germs on top of the bottle that we dont want to get in the vial because what is inside the vial is considered sterile

hence, addition of intrinsic factor in the second step results in an increase in vitamin B 12 absorption (over the baseline established in the first)

There are in general three reasons for this ignorance of the prevalence of common diseases , the lack of specificity of particular drugs for particular diseases, and the lack of standardisation of therapy