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(2014) reported that at short-term, B deficiency affects cytosolic Ca 2+ levels, and in roots, upregulates the expression of genes from the MYB protein family involved in Ca 2+ signaling and represses genes of the bZIP protein family with roles as channels/transporters, sensor relays and responders that act as intermediaries in a transduction pathway triggered by B deficiency, with important consequences in plant development, growth, flower maturation and stress (Zhao et al., 2021b)

This diagnosis was made on symptoms as my b12 was within range on the labs

Inhibition assay of cysteine cathepsin proteases The cathepsin B inhibitor screening assay 57 uses the ability of cathepsin B to cleave the synthetic AMC (7-amino-4-methylcoumarin)-based peptide substrate to release AMC, which can be quantified using a fluorometer or fluorescence microplate reader