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R 1 , R 2 , R 3 and R 4 are each independently selected from hydrogen, -OH, -NR 6 R 7 , halogen (e.g., -F, -Cl, -Br or -I), C1-4 alkyl or C1-4 alkoxy

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In laboratory and pre-clinical research settings, 5-Amino-1MQ is used as a molecular probe to investigate the consequences of NNMT inhibition across multiple metabolic and cellular systems

To further investigate the involvement of TRPM7-mediated Zn 2+ in pyroptosis, we measured the changes of pyroptosis associated factors in empty vector-transfected N2a cells, TRPM7-overexpressed cells after PILO treatment, and TRPM7-overexpressed cells after exposed to PILO combined with TPEN, a membrane-permeable Zn 2+ chelator