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Gradient conditions started at 1% B, increased linearly to 50% B over 7 min, then further to 100% B over 2 min, followed by a hold at 100% B for 6 min before returning to 1% B over 2 min

In the cytochrome P450 monooxygenase system, NADPH provides the reducing equivalents required by this series of reactions (Figure 13.7)

106 Glucokinase regulatory protein The glucokinase regulatory protein ( GCKR ) gene encodes glucokinase regulatory protein and is expressed mainly in the liver, pancreas, and colon

Floro-Styryl-Benzene (FSB) (Dojindo, Tokyo, Japan) staining was carried out according to the manufacturer's instructions