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Near-term perspectives: AI and rational design of geroprotective peptides Application of artificial intelligence to peptide design transforms the geroprotective research landscape

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A synthetic gene encoding pClcXGR3 (R 25 Q 380 ), featuring an eight-residue deletion (Y 78 Y 85 ) and 60 point mutations (R 25 K, S 26 T, I 27 L, Q 28 N, R 30 E, A 32 S, K 36 R, T 41 S, V 49 M, L 65 K, H 69 P, T 70 S, P 75 G, S 77 G, E 89 K, P 91 S, N 95 S, K 100 E, G 101 V, T 111 S, L 113 V, K 117 R, N 161 T, N 166 D, G 167 K, S 182 T, S 183 N, T 215 S, T 217 S, T 230 V, Q 241 K, V 244 T, G 247 A, Q 248 E, G 271 S, T 272 V, G 279 S, S 284 T, N 287 E, T 304 S, F 306 Y, T 320 R, K 325 R, C 334 V, V 335 T, T 337 E, Y 338 R, N 340 Q, V 341 T, S 343 T, T 345 P, A 346 T, N 347 E, S 348 K, N 357 Q, N 360 K, Q 362 E, Q 365 E, S 367 K and S 368 K), was obtained from GenArt (TFS) and cloned into pCMV-Sport6 via Eco91I and AsiSI sites, preserving the same flanking sequences as pS6-proNEP and pS6-pro-celiacase