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The choice to analyze the intracellular protein concentrations of IL-1B and CCL3 was based on the premise that the inhibition of gene transcription in response to B12 added to the turnover of these proteins by the proteasome machinery present inside the cells would result in detectable reductions in their concentrations after 24 hours of culture incubation

Similarly, impaired type I interferon activity and exacerbated inflammatory responses were also described in patients with severe COVID-19 (46), although progressive increases in disease severity correlated with transcript levels of JAK1, STAT1 and STAT2, interferon-2, interferon- receptors 1 and 2, and interferon regulatory factors 1, 4, 5, and 7