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Description

The pull-down method relies on the immobilization of a GST fusion protein on glutathione sepharose beads that serve as a solid phase

doi:10.1073/pnas.0502738102

The wells were dried at 37C for 30 min followed by adding 1,000 L of 80% ethanol (to dissolve crystal violet) and incubated further for 60 min at 37C with shaking (100 rpm)