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The U-PLEX Biomarker Group 1 (mouse) assay platform (Meso Scale Discovery, Gaithersburg, MD, USA) was used to measure interferon gamma (INF), interleukins (IL-1, IL-4, IL-6, IL-10, IL-12/23p40, IL-17A, and IL-22), keratinocyte-derived chemokine (KC), tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF), matrix metalloproteinase-9 (MMP9), chemokine protein known as regulated on activation normal T cell expressed and secreted (RANTES), interferon gamma-induced protein-10 (IP10), monocyte chemoattractant protein-1 (MCP1), and macrophage inflammatory proteins (MIP1, MIP2, and MIP3)

Researchers should confirm the product concentration, container information, compatibility, required handling controls and storage conditions from the supplied label, supplier documentation and their institutional protocol

Exploratory preclinical cancer research