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Then incubated in a 0.1% LFB solution (Sigma #1002798699) more than 8 hours at 56C

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The results indicated that Pc positively bound on zfh2 , vnd gene loci and did not bind on mthl10 , prosbeta2 gene loci in clock neuron clusters (Fig 7AT), whereas Pc positively bound on all four loci in non-clock neurons (Fig 7AT)