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In males with normal enzymatic activity, intratesticular testosterone concentrations are approximately 600ng/mLwell in excess of the average serum testosterone concentration (5ng/mL)

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After rinsing with PBS, cells were incubated with the secondary antibody (polyclonal, Goat anti-rabbit 1: 300, Alexa Fluor 488, Thermo Fisher Scientific, Waltham, USA) for 30 min, protected from light, at room temperature and under gentle stirring for homogenization