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7b), suggesting that the mTAD variant could still hetero-oligomerize with other PIFs

Proteins were resolved by nonreducing SDSPAGE, and revealed by western blot with 9E10 antiMyc monoclonal (a gift from C Crminon, Saclay, France), antiPdi1 rabbit polyclonal (a gift from J Winther, Denmark), rabbit polyclonal antithioredoxin (a gift from C Grant, UK) or antiHis antibodies, antimouse IgG or antirabbit IgG secondary antibodies labelled with fluorophores of different wavelengths, followed by Infrared Imaging technology (Odyssey, LICOR)