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Cells were cultured in basal medium (advanced DMEM/F12 with 10 mM HEPES, 1x Glutamax, and Penicillin/Streptomycin of 100 U/mL) supplemented with B27 (Invitrogen), 1 M N-acetylcysteine (Sigma-Aldrich), 10 nM gastrin (Sigma-Aldrich), 50 ng/mL mEGF (PeproTech), 50 ng/mL rhHGF (Bio-Techne R&D), 100 ng/mL FGF-10 (Peprotech), 1% R-spondin 3 conditioned medium, 10 mM nicotinamide (Sigma-Aldrich) and 10 M Rho-kinase inhibitors (AbMole)

Recent scientific research indicates that molybdenum, a trace mineral that activates the enzymes aldehyde oxidase and sulfite oxidase, may provide necessary nutritional support for chemically sensitive individuals

The functions of micronutrients are so vast that it would be impossible to list them all here

Ingredientes Celulose microcristalina [1] , Glutatio reduzido (G-SH) [3] , Hidroxipropilmetilcelulose [2] Enchimento invlucro da cpsula Setria uma marca registada da Kyowa Hakko Bio Co., Ltd